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CH2-Genome editing and transfer into animals - AI3 2025
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mcroze
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circular DNA that can be used as vector for cloning
Gene coding for beta-galactosidase and used as selection marker on some cloning vectors
color of undesired bacteria clones obtained with the selection marker of [2]
transfer method based on the use of electrical pulses to generate transient pores in the cell membranes
transfer method ideal to transfect plant cells or other cell types with rigid cell wall
Abreviation of the DNA damage generated by nuclease based methods of genome editing
repeated sequences between spacer sequences in bacteria's genome that are part of its "immune system"
Nuclease that uses a guide RNA to recognize and cut a specific 20 nt DNA sequence
Type of Recombination occurring naturally in cells and derivated to produce knock-out animals
qualifies a whole body KO or KI
short nucleotide sequences recognized by Cre recombinase
drug that can be used to induce a KO
Cre gene is under the control of a cell-specific one in some conditional KO model
qualifies an allele/gene that is flanked by two 11
qualifies embryonic stem cells potency
one of the popular method of DNA transfer to animals that can be used for large pieces of DNA
treatment that can be used to select transfected cells
can be used to transfer a transgene into an 8-cell embryo
type of mutation that can be generated by NHEJ